Unique Protein Moieties for 30 S and 50 S Ribosomes of Escherichia coli

Unique protein moieties for 30S and 50S ribosomes of Escherichia coli.

Each major component of the proteins of 30S ribosomes from Escherichia coli was compared with the proteins of 50S ribosomes. The comparisons were done by using polyacrylamide gel electrophoresis in urea with differentially labeled proteins. The data show that no major protein is common to both ribosomes.

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Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli.

RNA-protein cross-linked complexes were isolated and purified to obtain precise data about RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli. N-terminal microsequencing and matrix-assisted laser desorption ionization MS were used to identify the cross-linking sites at the amino acid and nucleotide levels. In this manner the following contact sites of five ribosomal pro...

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Assembly of Escherichia coli 50 S Ribosomes from Ribonucleic Acid and Protein Components I. CHEMICAL AND PHYSICAL FACTORS AFFECTING THE CONFORMATION OF ASSEMBLED PAR-

The in vitro assembly of Escherichia coli 50 S ribosomes from the ribonucleic acid and protein components of 50 S ribosomes was attempted. The two components were prepared by subjecting 50 S ribosomes to LiCl-urea treatment. The ribonucleic acid fraction contains 23 S RNA and 5 S RNA in equimolar amounts and is free from significant amounts of protein. The protein fraction is composed of all of...

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Assembly of Escherichia coli 50 S Ribosomes from Ribonucleic Acid and Protein Components I. CHEMICAL AND PHYSICAL FACTORS AFFECTING THE CONFORMATION OF ASSEMBLED

The in vitro assembly of Escherichia coli 50 S ribosomes from the ribonucleic acid and protein components of 50 S ribosomes was attempted. The two components were prepared by subjecting 50 S ribosomes to LiCl-urea treatment. The ribonucleic acid fraction contains 23 S RNA and 5 S RNA in equimolar amounts and is free from significant amounts of protein. The protein fraction is composed of all of...

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Assembly of Escherichia coli 50 S ribosomes from ribonucleic acid and protein components. I. Chemical and physical factors affecting the conformation of assembled particles.

The in vitro assembly of Escherichia coli 50 S ribosomes from the ribonucleic acid and protein components of 50 S ribosomes was attempted. The two components were prepared by subjecting 50 S ribosomes to LiCl-urea treatment. The ribonucleic acid fraction contains 23 S RNA and 5 S RNA in equimolar amounts and is free from significant amounts of protein. The protein fraction is composed of all of...

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Unique Protein Moieties for 30 S and 50 S Ribosomes of Escherichia coli

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